Same comments apply here as with ab32077
Images
EGFr in human TR146 buccal epithelial squamous cell carcinoma line : FFPWS using Abcam ab32077
Antibody should be further diluted ( used at 1/400)
Cells were both scraped or trypsinised ( in case enzyme altered signal), spun down. Resuspended in 10 % Formalin O/N at RT.
Spun down, rinsed/resuspended in TBS.
After another spin, cell pellet was resuspended in HMP Agarose at 50C and then immediately spun down at 4K for 5 mins.
Agarose gel containing pellet was processed to Pwax conventionally.
Is this positivity specific?
It appears to be identical to that seen using ab40815 and also ab52894
Sure, one sees membrane positivity.
EGFr in human placenta: FFPWS using Abcam ab52894
EGFr in human placenta: FFPWS using Abcam ab32077
I am not familiar with EGFr positivity/distribution within any tissues under these conditions.
However, HPA shows an identical positivity under similar conditions. Abcam’s ab52894 shows a very similar distribution pattern.
However, Abcam’s ab40815 (anti pEGFr) is negative.
Comments always welcomed
🙂