Meningial area: these bvs are outside of the brain therefore will be better fixed. Also, there is no ( minimal) hydrophobic lipids to impede fixation. So, this protein appears to be robust enough to withstand autolysis due to PM delay in fixation?
NB: positivity is strong, punctate in the bvs’ smooth muscle cytoplasm
Poor results compared with the surgical kidney specimen ( I had to use 1/100 instead of 1/200 dilution factor). This is because of the delay in fixation that is routine in PM brains. Also, rarely are the brains, upon dissection, dissected into portions that facilitates better fixation. One can easily tell if the brain ( or any other tissue) is sub-optimally fixed by looking for blood vessels. They will appear detached from the neuropil.
Medulla: strong punctate collecting tubule positivity. Also similar positivity in renal artery smooth muscle cells.
Well…all cells are positive!
Good positivity at 1/250 dilution factor.
Excellent positivity at 1/1K dilution factor.
Mouse brain. Too strong at 1/1000. Can be diluted to 1/2K and still get strong positivity.