Positivity appears restricted to basal laminae ( particularly evident in the T/S presentation of smooth muscle cells of the inner layer of the muscularis externa : this is a longitudinal orientation of the specimen so, the inner circular layer shows as T/S)
The intense positivity seen in cells within the lamina propria is due to endogenous IgG ( Plasma cells) as I forgot to use rat-adsorbed anti mouse IgG secondary antibody.
FFPWS of rat brain. I probed with anti Iba1 ( Wako Rb) then used within date Gt anti Rb-Biotin.
Which section has used a stABC-px kit within Use-by date and which section has used a kit that expired in 2012?
Sweat glands and duct in dermis.
Positivity is seen at the cell-cell adherens junctions of the epidermal cells.
Positivity is seen at neuromuscular junctions
Components of Auerbach’s plexus are positive ( ganglia and individual intramuscle nerve fibres)