Immunopositive platelets are seen in the red pulp.
Unfixed frozen section of mouse lung. Sections cut at 10 microns, mounted onto slides and dried overnight in a fumehood “on”.
Upper image : Formalin-fixed for 15 mins at RT
Lower image: Acetone – fixed for 15 mins at RT.
Note the FIF in the upper image. I am surprised that there is FIF even with such a short fixation time.
Arrowheads indicate negative Purkinje cells.
As in all species I have tested , Purkinje cells of the cerebellum are always negative for this Ab.
Arrowheads indicate the negative rostro-migratory stream ( RMS) of the progenitor olfactory neurones.
Note the positive post-mitotic neurones and the negative progenitor olfactory interneurones in the svz of the lateral ventricle.
All neurons, as far as I am aware, are HuC/D positive.