Same method as in FS of rat bladder
I overstained the tissue section and then dipped it into 0.5% HCL in 20% IMS to differentiate the staining intensity until I was happy with the signal: noise.
It is a tad more laborious than using more highly selective methods but, serves a purpose.
At pH1 Alcian blue positivity is limited to highly acidic glycosaminoglycans (heparin/ chondroitin/dermatan/keratan sulphates, that I understand).
At pH3 Hyaluronic acid-containing molecules are also positive. At pH0.5 only dermatan/keratin sulphate are positive.
One may alternatively use the CEC method to demonstrate the various “mucins”, to a high degree of selectivity.
Both methods rely on charge densities, that I understand.
Identical positivity to that seen in rat cerebellum.
Interestingly, the most intense positivity is seen on the membrane of the soma of Purkinje cells; this extends a short distance along the primary dendrite and then appears to cease to be expressed.
Positivity on the membranes of the Purkinje cell secondary dendrites and also intense positivity apparently on the membranes of the Purkinje cell bodies
Pattern of positivity is seemingly identical to that seen in human spleen.
Is the cytoplasmic positivity seen in sinusoid -lining cells specific?
Pattern of positivity is identical to that shown by HPA. Intensely stained individual cells are scattered throughout the white/red pulp.
However, cells lining the sinusoids also appear positive
Positivity is not as striking as in mouse kidney.
Cortex: intense brush border in a subset of convoluted tubules. A moderate cytoplasmic positivity in most tubules.