NCAD is enriched in svz and migrating olfactory interneurons ( in the RMS).
Note that capillary positivity is due to plasma IgGs and not NCAD.
( the brain was not perfused)
There is a moderate cytoplasmic positivity.
Immunostaining intensity is far too strong as I used a dilution factor that is optimal for my homemade DAB solution but, I developed using a commercial, enhanced- DAB kit.
However, an interesting pattern of positivity.
I would appreciate comments/explanations.
I am not yet familiar with the distribution pattern of this protein. However, published data states that this protein is present in the nuclei of gut mucosal epithelium ( embryonic and adult).