Images

TFAM in human PM brain: FFPWS using Abcam ab171951

1in100b

Meningial area: these bvs are outside of the brain therefore will be better fixed. Also, there is no ( minimal)  hydrophobic lipids to impede fixation. So, this protein appears to be  robust enough to withstand autolysis due to PM delay in fixation?

NB: positivity is strong, punctate in the bvs’ smooth muscle cytoplasm

TFAM in human PM brain: FFPWS using Abcam ab171951

1in100a

Poor results compared with the surgical kidney specimen ( I had to use 1/100 instead of 1/200 dilution factor). This is because of the delay in fixation that is  routine in PM brains. Also, rarely are the brains, upon dissection, dissected into portions that facilitates better fixation. One can easily tell if the brain ( or any other tissue) is sub-optimally fixed by looking for blood vessels. They will appear detached from the neuropil.