Positivity is not as striking as in mouse kidney.
Cortex: intense brush border in a subset of convoluted tubules. A moderate cytoplasmic positivity in most tubules.
Interface of keratinising/mucosal regions.
T/S at region of dorsal root entry.
Intense cardiomyocyte positivity.
Smooth muscle cells of oesophagus are also positive.
T/S showing entry zone of dorsal root.
Strong immunopositivyt in nerve fibres in the root and of neuropil in the spinal cord.
Note an intense positivity in the most superficial lamella.
Intense positivity in small ganglion cells; nerve fibres are strongly positive. NovaRed chromogen ( Vectorlabs).
This chromogen gives a more intense positivity than std DAB ( thus one can dilute out the primary antibody x2, imho).
Sure, adding Imidazole to std DAB gives ~ x3 intensity.