As in all other anti Dnmt3a antibodies I have tested, there is strong nuclear positivity in ?spermatogonia? cells. A moderate cytoplasmic positivity is seen.
Strong nuclear positivity is shown in the ?spermatogonia” layer.
There is a moderate positivity in nuclei of many developing sperm cells.
There is also a moderate cytoplasmic positivity, most obvious in fully formed sperm cells.
HPA shows strong nuclear positivity throughout the layers.
I only get the ? spermatogonia? nuclei immunostaining.
Nerve fibres and Nerve plexi are positive ( shown is one of Auerbach’s plexus)
See Abcam Ab PGP9.5 image: identical positivity.
I recommended this Ab to a colleague. He ordered it….got no reply ( from Ultraclone). He persisted….they stated that they had no more Ab….buy it from Abcam!
( I apologise for any paraphrasing )
Ventral aspect of the spinal column. Not only are the cord nerve fibres positive; what seem to me to be osteoclasts, in the vertebra, are also positive( black arrowheads). They are also positive in an Abcam ab.
The IofL is disrupted. Black arrowheads indicate individual nerve fibres running alongside a blood vessel.
Fine fibres are seen but, as expected, they are easier to visualise when using IF.
Most of the IofL cells are positive. A few are remarkably negative.
Black arrowheads indicate positivity seen in fine nerve fibres running along a blood vessel.
Detection of intra-epidermal nerve fibres: anti Rb Alexa 594. 8 micron section.