Specific? Strong positivity in Basket cell cytoplasm.

I do not know if the positivity I obtain using this antibody on Formalin-fixed Pwax sections, after HIER, is specific.

Similar pattern of positivity to that seen in human kidney ( arrowheads point to individual strongly positive nerve fibres) although the background, non-specific positivity is much higher

The kidney itself is not positive( well, there is a slight background non-specific positivity that should disappear when the primary antibody is diluted further) but individual nerve fibres are clearly positive ( indicated by black arrowheads).

I do not know the specificity of these results. Abcam datasheet includes “…..plays also a key role in the
maintenance of axonal homeostasis, the prevention of axonal degeneration…”

Abcam datasheet does not state IHC-P reactivity.

Positivity is confined to neuronal cytoplasm/processes in central and peripheral nervous system.

Image shows such positivity in nerve fibres and also Auerbach’s plexus neurone cell cytoplasm

I did the initial histology and IHC for a student to establish the “best marker” to identify the cells if they were found in the heart. Once we agreed that ab190710 gave the best results ( I also tested anti TFAM,  MTCO2 and STEM121 : all human -specific) the student carried on without aid. I also probed with anti GFP as the progenitor cells were labelled with GFP. They all worked but gave an inferior signal:noise

The PhD student: I injected human cardiac progenitor cells (c-kit+, CD31-) isolated from human heart tissue into NSG mice.

Positivity in progenitor olfactory inter neurones. SVZ of lateral ventricle.