An older patient who thus, most sadly, hadn’t been diagnosed. So, a massive proliferation of compensatory nerve fibres that are useless?
A functional technique, determining that the macrophages are activated. Immunomethods using anti CD68/Ox42….would be the modern equivalent?
An early clot, noticed by chance.
Acidophilic inclusion bodies in cells at the base of crypts of Lieberkühn.
Predominantly in stratum spinosal cells. Note that glycogen is assymetrically distributed within each cell ( black arrowhead). This is because of the unidirectional penetration/flow of fixative when a specimen is placed in fixing fluid without agitation: the glycogen is pushed ahead of the wave of fixing fluid until it cannot go further because of the cell membrane.
Melanin within melanocytes is indicated by a yellow arrowhead. After transportation into cells of the basal layer ( red arrowhead), melanin forms a cap over the nuclei ( protecting DNA from UV light…up to a point!).
Poor quality of tissue and counterstain ( Nuclear fast red) is because this was a post-mortem specimen.
Technique is not specific for Candida but, taking into account important data (eg: an immunocompromised patient) ,the morphology aids the diagnosis ( no pun intended).