CRABP1 in mouse brain – FFPWS using abcam ab2816

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Arrowheads: SVZ of lateral ventricle ( black); direction of migratory progenitor olfactory interneurons ( red) ; intensely positive cells within the striatum ( green).

Any ideas what these cells are? ( HPower image also submitted).

Positivity appears to be localised to this region. Is this positivity specific? Abcam no longer sell this reagent.

Caspr2 and GFP in HEK293 cells: FFPWS using abcam ab137052

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Upper image shows GFP tag ( using anti GFP ab13970) in same cells as anti Caspr. However, the transfection rate is poor. Also, there is anti Caspr positivity in cells that do not express GFP.

I am not sure what this means: advice is always appreciated

Cells were fixed in 10% Formalin in suspension and finally spun down in 1% HT Agarose. The set agarose block was then processed to Pwax, sections cut and mounted onto slides. After HIER, sections were co-incubated in anti GFP/Caspr2.