Note the extensive arborisations of the indicated ( red arrowhead) astrocyte ( I assume). The cytoplasm is not positive. Sure, many of the top R and L processes are most likely other cells’.
Also note the intimacy with the neurone ( blue cytoplasm, unstained nucleus, excepting the nucleolus).
Lateral ventricle: intense positivity in svz cells ( appears to be nuclear and cytoplasmic: ependymal cells most obviously show the nuclear positivity)
The antibody is either of low sensitivity or the protein is very low copy-number because the positivity is moderate using a std stABCpx-DAB detection ( lower image). Using Nova red kit increased the signal significantly
Of course, one may use the higher sensitive DAB kits or, add imidazole to your DAB solution.