Datasheet states human- reactive. ( only?). Clearly, mouse macrophages are positive.

Macrophages are positive ( all or only activated ones?)

50 micron free-floating cryosections were defrosted and processed tp Pwax. There is fine neurofibre positivity.

Snap-frozen sample of Formalin-fixed cord ( injected with mCherry tagged molecule ) was defrosted and processed to Pwax. 6 micron sections were cut and probed for mCherry

The antibody is conjugated to Alexa 647: I used this because it was the only ab available from the Researcher. For IHC, use the unconjugated antibody.

C3d is positive for epididyimal epithelium; Clusterin is positive for ECM around epidydidimal tubules. Note that “functioning” tubules have a single Clusterin positivity but, senescing tubules are multi-layered. Senescent tubules seem to become singular wrt Clusterin

Comments?

Compare this result with same field, using Abcam ab69644 ( anti Clusterin). Interestingly, C3d is positive for the senescing seminiferous tubules’ cytoplasm but the “disorganised” Clusterin expression ( compared with non-senescing tubules) is still limited to the connective tissue tunica ( ECM) of the tubules. I do not know of the significance of this, if there is one!

Astrocytes can be seen to be surrounding an amyloid plaque

Comparing expression pattern in normal ( upper image, showing dorsal root entry zone) and traumatic injury specimen ( lower).

Excellent positivity in microglia, only