The datasheet states that it has been reported to x-react with eNOS and also nNOS. On FFPWS after Citric acid HIER I concur. Submitted images shows clear endothelial positivity, I am not sure what the heavily positive cells in the marginal zone of the white pulp are ( also some around high endothelial venules).
Compare pattern of positivity using ab34710 ( anti Collagen type I) on sequential section.
Excellent immunopositivity of all post-mitotic neurones, using Alexa 594 as reporter molecule
Lateral ventricle showing non- immunostained progenitor ollfactory interneurones ( stained blue by Haemalum) in the svz.
Post-mitotic neurones in the striatum are positive ( brown, using DAB as visualising agent)
Specific? Strong positivity in Basket cell cytoplasm.
I do not know if the positivity I obtain using this antibody on Formalin-fixed Pwax sections, after HIER, is specific.
Similar pattern of positivity to that seen in human kidney ( arrowheads point to individual strongly positive nerve fibres) although the background, non-specific positivity is much higher
The kidney itself is not positive( well, there is a slight background non-specific positivity that should disappear when the primary antibody is diluted further) but individual nerve fibres are clearly positive ( indicated by black arrowheads).
I do not know the specificity of these results. Abcam datasheet includes “…..plays also a key role in the
maintenance of axonal homeostasis, the prevention of axonal degeneration…”
Abcam datasheet does not state IHC-P reactivity.
Positivity is confined to neuronal cytoplasm/processes in central and peripheral nervous system.
Image shows such positivity in nerve fibres and also Auerbach’s plexus neurone cell cytoplasm
I did the initial histology and IHC for a student to establish the “best marker” to identify the cells if they were found in the heart. Once we agreed that ab190710 gave the best results ( I also tested anti TFAM, MTCO2 and STEM121 : all human -specific) the student carried on without aid. I also probed with anti GFP as the progenitor cells were labelled with GFP. They all worked but gave an inferior signal:noise
The PhD student: I injected human cardiac progenitor cells (c-kit+, CD31-) isolated from human heart tissue into NSG mice.