Images

ATG7 in human colon: FFPWS using abcam ab133528

Hu1in3Kcabrev

I do not know the specificity of these results. Abcam datasheet includes “…..plays also a key role in the
maintenance of axonal homeostasis, the prevention of axonal degeneration…”

Abcam datasheet does not state IHC-P reactivity.

Positivity is confined to neuronal cytoplasm/processes in central and peripheral nervous system.

Image shows such positivity in nerve fibres and also Auerbach’s plexus neurone cell cytoplasm

Human nuclei in mouse heart : FFPWS USING Abcam ab190710

Nuclei-ab190-1in250

I did the initial histology and IHC for a student to establish the “best marker” to identify the cells if they were found in the heart. Once we agreed that ab190710 gave the best results ( I also tested anti TFAM,  MTCO2 and STEM121 : all human -specific) the student carried on without aid. I also probed with anti GFP as the progenitor cells were labelled with GFP. They all worked but gave an inferior signal:noise

The PhD student: I injected human cardiac progenitor cells (c-kit+, CD31-) isolated from human heart tissue into NSG mice.