Upper image shows GFP tag ( using anti GFP ab13970) in same cells as anti Caspr. However, the transfection rate is poor. Also, there is anti Caspr positivity in cells that do not express GFP.

I am not sure what this means: advice is always appreciated

Cells were fixed in 10% Formalin in suspension and finally spun down in 1% HT Agarose. The set agarose block was then processed to Pwax, sections cut and mounted onto slides. After HIER, sections were co-incubated in anti GFP/Caspr2.