T/S through body at thoracic level. Neuropil of spinal cord, ganglia and nerves are positive. Collagen ( ?tendinous?) around cartilage is also positive.
? Specific?
Excellent positivity under standard conditions ( Citra HIER/stABCpx-DAB). This ab is biotinylated so, using the std ABC technique I was able to achieve a high dilution factor ( 1/5K).
Should be diluted further, under these conditions.
I haven’t submitted an Abreview for this Ab as I forgot who gave me a sample ( so no lot number…doh!)
Striatum showing positivity for Ankyrin G in the initial segment of axons: enables concentration of voltage-gated sodium channels for the initialisation of the action potential.
I have no idea why there is a moderate neuropil positivity…is it non-specific?
This antibody is no longer available because Santa Cruz have had their rabbit/goat polyclonal antibody production licence revoked.
On a tangent, their Abs were termed “Santa Crap” for many years….unfairly, imho but, their early days marketing appeared to be more money-driven than is expected from any commercial/scientific organisation…. little did I know how badly, allegedly, they were treating their animals
I add the image as an example of Ankyrin G- positivity.