Monthly Archives: June 2015
MHC in mouse gastrocnemius muscle: Frozen section using DSHB A4.951
MHC in mouse gastrocnemius muscle: Frozen section using DSHB A4.840
DSHB is an excellent source of antibodies….not for profit!
MHC in mouse gastrocnemius muscle: Frozen section using DSHB A4.74
MHC in mouse gastrocnemius muscle: Frozen section using Chemicon MAB1628
MHC in mouse gastrocnemius muscle: Frozen section using DSHB N2.261antibody
Placoid scales in catfish: H&E of GMA section
T/S towards tail showing two scales ( also known as dermal denticles).
Macrophages in adult mouse liver: FFPWS using anti CD68 ab clone FA-11 and Abcam anti IBa1 ab107159
I tested AbDSerotec’s and Abcam’s FA-11 clone. Neither were positive using conditions that gave excellent positivity on sequential section, using anti Iba1 ( ab107159).
Upper image is anti CD68 clone FA-11.
Lower image is anti Iba1.
So, in my hands, clone FA-11 anti CD68 is negative for “IHP”.
Also, it is stated in AbDSerotec’s datasheet that CD68 is a Pan-macrophage marker. That is not my experience.
Iba1 is a pan-macrophage marker.
Comments very welcome.
cd26 in e18 mouse thymus : FFPWS using R&D systems AF954
Not a good image but, it shows that a large proportion of cells within the parenchyma of the thymus are positive.
cd26 in e13 mouse gut : FFPWS using R&D systems AF954
Literature supports this positivity as specific but, I have no previous experience with this protein.
As well as the fibroblast capsule positivity, there is also strong immunostaining of the gut lining epithelium.