Osmium preparation of sciatic nerve: FFPWS

SNAP-083900-0001

Sciatic nerve was fixed in 10% Formalin then placed in 1% Osmium for 1hr with gentle agitation. After rinsing well in dist. water the specimen was processed to Pwax. 8 micron section mounted onto subbed slides ( I found that the sections did not adhere well to Superfrost Plus nor PLL – coated slides.Large, intensely black “blobs” are individual adipocytes.