Mouse spleen.

Upper image after M/W ( 1/2000). Should be further diluted.

Lower image after 90C AR for 60mins ( 1/1000)

M/W gives superior results and a higher dilution factor.

However, AR at 90C gives an identical pattern of positivity.

AR at 90C for 30mins gave an identical pattern but, was approx. half as intense as 60 mins AR ( not shown)