Mast cells in rat bladder: FS using 1% Toluidine blue ( aq)

FS-RatTolblue-a

I overstained the tissue section and then dipped it into 0.5% HCL in 20% IMS to differentiate the staining intensity until I was happy with the signal: noise.

It is a tad more laborious than using more highly selective methods but, serves a purpose.