Iba1 and Heme oxygenase in mouse spleen: FFPWS using Wako 019-19741 and Abcam ab85309 Image April 7, 2017 Carl Hobbs Anti Iba1 ( top image) is positive for all ( I assume) macrophages. Heme oxygenase ( lower image) seems to be positive only in Haemoglobin- catabolising macrophages in the red pulp.