CD31 in human kidney: FFPWS using Abcam ab76533

Abcam1in200TRISpH9EDTA

I used TRIS pH9+EDTA HIER solution.

Identical positivity after TRIS pH9 ( no EDTA).

After Citric acid pH6 HIER, positivity was identical.

However, background noise was seen ( decreased Signal:noise)