This antibody should be further diluted as the grey matter positivity may be non-specific. However, I am using a dilution factor of 1/20K.
I find the Dorsal column ( lower part only) interesting but, I have no idea why it is confined to this area ( something to do with ascending nerve fibres crossing over?).
Midbrain adjacent to cerebellum: there is a complete absence of Neurogranin in rat cerebellum ( indicated by negative Molecular and Granule cell layers in submitted image
