Note fine fibres crossing the basal lamina into the epithelium.

Localised to adherens junctions of intercalated discs ( shown)and also Purkinje fibre plasma membranes (not shown)

Injured SN site. Positive microglia ( ramified) and possibly a few incoming macrophages ( amoeboid) from the blood vessel to the left of the image.

The datasheet states that it has been reported to x-react with nNOS and also eNOS. On FFPWS after Citric acid HIER I concur. Submitted images shows clear neuronal positivity ( SN compacta cells ).

The datasheet states that it has been reported to x-react with eNOS and also nNOS. On FFPWS after Citric acid HIER I concur. Submitted images shows clear endothelial positivity, I am not sure what the heavily positive cells in the marginal zone of the white pulp are ( also some around high endothelial venules).

Compare pattern of positivity using ab34710 ( anti Collagen type I) on sequential section.

Excellent immunopositivity of all post-mitotic neurones, using Alexa 594 as reporter molecule

Lateral ventricle showing non- immunostained progenitor ollfactory interneurones ( stained blue by Haemalum) in the svz.

Post-mitotic neurones in the striatum are positive ( brown, using DAB as visualising agent)