Top: CD68

Middle: Iba1

Bottom: MMP9

Rat cannulation injury site.

Comments welcome re the fact that MMP9 appears to be positive  in a subset of CD68 positive macrophages.

Antibody should be further diluted ( 1/3K used). Positivity appears confined to macrophages of the red pulp sinusoids.

Germinal centres show positive dendritic macrophages ( black arrowhead)

Rounded, smaller +ve cells are seen in the high endothelial venules ( red arrowhead) Are these neutrophils?

In the muscularis externa positivity is seen only in the nuclei of

?satellite cells? associated with the ganglion cell bodies of

Auerbach’ s plexus

See sequential section image immunolabelled for Islet 1 ( 40-3A4)

See sequential section immunolabelled for Nestin ( 401)

Hippocampal region. Reagent no longer sold by Abcam but image retained to find out if anyone knows if this pattern of positivity is specific ( HPA give their Abs enhanced orthogonal rating yet their brain images give an entirely different positivity)

Frontal cortex.

I am not familiar with this protein: comments welcome.

L/S thro medulla showing positive vasa recta endothelium