The pattern of distribution and the shape of the most obviously positive cells leads me to conclude that they are macrophages.

Comments welcome.

Wt lung.

If I am correct, there are two populations of positive cells in the alveolar spaces: one is smaller more rounded and intensely positive. The other is slightly larger, irregular, with a seemingly membraneous lighter positivity.

There is also a moderate positivity apparently lining the alveoli.

Non-specific ( antibody should be diluted more)

.

Sciatic nerve was fixed in 10% Formalin then placed in 1% Osmium for 1hr with gentle agitation. After rinsing well in dist. water the specimen was processed to Pwax. 8 micron section mounted onto subbed slides ( I found that the sections did not adhere well to Superfrost Plus nor PLL – coated slides.Large, intensely black “blobs” are individual adipocytes.

Muscularis externa: there is a punctate positivity at the membranes of the smooth muscle cells.

Specific? Spurious?

As always, any explanations would be appreciated.

Stated as being rat, mouse and human reactive but, the distribution of positivity is odd, to me.

However, GeneAtlas RNA expression data gives high levels for this protein, in colon.

A range of positive cells( arrowheads indicate such):

red for endothelium; green for ? fibroblasts; black for cells in the basal area of the epidermis – processes of these cells are seen; blue for a subset of inflammatory cells.

Eye region: black indicates nerve bundles; red indicates individual nerve fibres within the developing extra-ocular muscles.

Developing naso-pharyngeal epithelium

Developing eye.