Auerbach’s plexus; ganglion cell bodies, associated nerve trunks and individual fibres in both smooth muscle layers are clearly demonstrated.

Arrowheads indicate positivity: black and green for nerve fibres; red for ganglion cells ( Meissner’s plexus).

In white matter areas many fibres have a punctate positivity

Lewy bodies clearly seen, as well as   occasional fibres.

Meningial area: these bvs are outside of the brain therefore will be better fixed.This protein appears to be  robust enough to withstand autolysis due to PM delay in fixation?

NB: positivity is strong, punctate in the bvs’ smooth muscle cytoplasm

Poor results compared with the surgical kidney specimen ( I had to use 1/100 instead of 1/200 dilution factor). This is because of the delay in fixation that is  routine in PM brains. Also, rarely are the brains, upon dissection, dissected into portions that facilitates better fixation. One can easily tell if the brain ( or any other tissue) is sub-optimally fixed by looking for blood vessels. They will appear detached from the neuropil.

Medulla.

Medulla: strong punctate collecting tubule positivity. Also similar positivity in renal artery smooth muscle cells.

Well…all  cells are positive!