Coronal section through adult: 3rd ventricle showing intense luminal membrane positivity of ependymal cells. I do not know what the positive cells are, immediately what appears to be the corpus callosum. A less intense neuropil positivity is seen.

Coronal section through adult: 3rd ventricle showing intense luminal membrane positivity of ependymal cells. I do not know what the positive cells are, immediately what appears to be the corpus callosum. A less intense neuropil positivity is seen.

I was titrating a totally different primary Antibody and had a spare section so, I probed it with this primary Ab.

Strange that Islet of Langerhans cells are positive?

Non-specific?

Primary antibody should be further diluted ( 1/5K used ).

Dermal sweat glands/ducts.

Further dilution required

Developing epidermis. The primary antibody needs further dilution, using my std stABCpx-DAB detection system.

Developing oesophagus: primary Ab needs to be further diluted, using my std stABCpx-DAB method.

No membrane positivity is seen. However, many nuclei are positive. Non-specific?