Macrophages ( Microglia) specifically express GFP, in this transgenic mouse. Note that rbcs are positive for Alexa 488; this is due to formalin-induced fluorescence, particularly strong in green wavelength but also in the visible red wavelength
HIER is best but Proteinase K is also good, for antigen retrieval. Anti NeuN ab is directly conjugated to Alexa Fluor 647. Chicken anti GFP was detected using Goat anti chicken-biotinylated ( Abcam) and then stAvidin-488 Invitrogen.
Intense positivity is seen in the bile ducts within the portal area.
Cells lining the sinusoids are also positive( ?Kupffer cells?)
Most intense positivity is seen in epithelium of respiratory bronchioles.
Intense positivity in respiratory epithelium of bronchioles.
Most intense positivity is seen in bile ducts of portal area.
Intensely positive cells surrounding capillaries.
Note that the capillaries are patent ( due to perfusion-fixation).
TASTPM mouse. Intensely positive cell ( ?astrocyte) appears to be closely associated with a plaque.
TASTPM brain. Positive cells are “surrounding” amyloid plaques
Substantia nigra?