NCAD in p5 mouse brain: FFPWS using BD 610920

Image February 28, 2015 Carl Hobbs

NCAD is enriched in svz and migrating olfactory interneurons ( in the RMS).

Note that capillary positivity is due to plasma IgGs and not NCAD.

( the brain was not perfused)

CDX2 in adult human colon : FFPWS using Abcam ab76541

Image February 22, 2015 Carl Hobbs

CDX2 in adult rat colon : FFPWS using Abcam ab76541

Image February 22, 2015 Carl Hobbs

There is a moderate cytoplasmic positivity.

Connexin 43 in mouse spleen : FFPWS using Abcam ab11370

Image February 13, 2015 Carl Hobbs

Immunostaining intensity is far too strong as I used a dilution factor that is optimal for my homemade DAB solution but, I developed using a commercial, enhanced- DAB kit.

However, an interesting pattern of positivity.

I would appreciate comments/explanations.

CDX2 in e14 mouse gut: FFPWS using Abcam ab76541

Image February 13, 2015 Carl Hobbs

I am not yet familiar with the distribution pattern of this protein. However, published data states that this protein is present in the nuclei of gut mucosal epithelium ( embryonic and adult).

CD10 ( CALLA, Neprilysin) in rat kidney: FFPWS using R&D systems af2736

February 11, 2015 Carl Hobbs Kings College London

Parietal cells of the glomerulus and also distal cells of the Macula densa ( DCT) are strongly positive.

CD10 ( CALLA, Neprilysin) in rat kidney: FFPWS using R&D systems AF2736

February 11, 2015 Carl Hobbs Kings College London

Medulla: given that the PCTs’ surface epithelium shows strong positivity ( in the cortex) I assume that the irregular intense positivity here is the descending ( thick) part of the Loop of Henle. Most other tubular structures ( whether collecting ducts/thin loops of Henle) show a moderate surface epithelial positivity.