Macrophages in adult mouse liver: FFPWS using anti CD68 ab clone FA-11 and Abcam anti IBa1 ab107159

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I tested AbDSerotec’s and Abcam’s FA-11 clone. Neither were positive using conditions that gave excellent positivity on sequential section, using anti Iba1 ( ab107159).

Upper image is anti CD68 clone FA-11.

Lower image is anti Iba1.

So, in my hands, clone FA-11 anti CD68 is negative for “IHP”.

Also, it is stated in AbDSerotec’s datasheet that CD68 is a Pan-macrophage marker. That is not my experience.

Iba1 is a pan-macrophage marker.

Comments very welcome.