I did the initial histology and IHC for a student to establish the “best marker” to identify the cells if they were found in the heart. Once we agreed that ab190710 gave the best results ( I also tested anti TFAM, MTCO2 and STEM121 : all human -specific) the student carried on without aid. I also probed with anti GFP as the progenitor cells were labelled with GFP. They all worked but gave an inferior signal:noise
The PhD student: I injected human cardiac progenitor cells (c-kit+, CD31-) isolated from human heart tissue into NSG mice.