Re: Frozen IHC
From: | antje.marcantonio@pharma.Novartis.com |
Jim,
I cut, dry overnight, then start IHC with acetone fixation (there are
antibodies which work better/only with paraformaldehyde fixation).
Slides I don't use right after drying I keep in the -80 freezer.
This is what we are doing now routinely, but I know it is not the only way
to get your IHC working. You just have to find out your best way.
Good luck.
Antje
Antje Marcantonio
Novartis Pharma AG, Basel
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