Katie is right about the usefulness of these slides. They are also available
as small T flasks if you only want one chamber on the slide. However,
remember that not all cells are adherent to glass. Some only grow on plastic
so the slides are available both with plastic slides and glass slides.
Unfortunately, the plastic slides don't standup to treatment with reagents
such as xylene so this must be factored into your procedure.

John Tarpley 15-2-B
Associate Scientist
Specialist Image Analysis & Immunohistochemistry
Amgen Inc
One Amgen Center Drive
Thousand Oaks, CA  91320

Views expressed are mine alone and do not represent the views of my employer


> ----------
> From: 	Bennett, Catherine (Katie)[SMTP:cbennett@lrri.org]
> Sent: 	Tuesday, October 19, 1999 9:17 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	RE: Staining Cell Cultures
> 
> I would recommend that you tell your client that he should grow the cells
> directly onto a slide using cell culture chamber slides.  These special
> slides have a little chamber that is attached directly onto the slide,
> allowing you to culture your cells right on the slide.  Once you are done,
> you just pop off the well walls and are left with the cells on the slide.
> Nalge makes a whole line of them and you should be able to find them in
> any
> VWR or Fisher catalog.  In a study I was involved in where we wanted to
> stain for actin filaments, we grew the cells up right on the slide until
> ~90% confluent, fixed the cells, then did the immunohistochemistry
> staining
> right in the cell well chambers.  We only removed the well wall after
> staining was complete, then coverslipped and analyzed on the scope.
> 
> *********************************
> Catherine "Katie" Bresee Bennett
> Sr. Technical Associate
> Lovelace Respiratory Research Institute
> Albuquerque, New Mexico
> 
> 
> > -----Original Message-----
> > From:	HistoScientific [SMTP:histosci@shentel.net]
> > Sent:	Tuesday, October 19, 1999 8:08 AM
> > To:	histonet@pathology.swmed.edu
> > Subject:	Staining Cell Cultures
> > 
> > Dear Netters,
> > 
> > We have a client that would like to see lipid vesicles in cell cultures
> > that he has developed in a Petri dish.  How do we get the cell culture
> > on the microscope slide?  Is the culture something you can frozen
> > section, then stain?  We work with the actual tissue most of the time,
> > therefore we are not very experienced with cell cultures!  Any
> > suggestions would be most appreciated.
> > 
> > Sincerely,
> > 
> > Beth Poole
> > Histo-Scientific Research Labs.
> > 107 Killmon Road
> > P.O. Box 30
> > Basye, VA  22810
> > (540)856-2222
> > fax: (540)856-2227
> > histosci@shentel.net
> > 
>