Hi Susie,

The bulk of my work is on paraffin embedded rat brain tissue sections.  I use
immunofluorescent detection systems all the time - alone or dual.  Our stuff
is fixed in 4% NBF overnight.  We have our troublesome antibodies, but for the
most part it works beautifully.  Good luck!

Angeline Meadows
Medical College of Georgia
meadows@neuro.mcg.edu

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