Re: Rapid Processing of Kidney Biopsies
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From: | "Barry Rittman" <brittman@mail.db.uth.tmc.edu> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Pamela,
I think that Robert's comments are right on track. I feel that one
of the major problems is that we are most comfortable with that which we know
best. When different fixatives are used it takes a lot of exposure before we
become comfortable with the different images. I remember the great resistance
to the use of frozen sections when reliable cryostats became commercially
available.This resistance was largely because of the different image that was
seen - with less shrinkage and distortion and with the retention of many
components. When monoclonal antibodies came into vogue suddenly the need for
cryostat sections increased dramatically and we all became used to examining
frozen as well as paraffin sections and were, after a time, able to compare
both.
If, as Robert suggested you provide your pathologists with some better
alternatives this may be the best way to go.
Barry
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