IHC on Glutaraldehyde Fixed Tissues
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From: | Paul Kwan <paul.kwan@es.nemc.org> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 08 Jun 1999 18:11:52 -0400 (EDT) |
Content-Type: | text/plain; charset=US-ASCII |
Hi,
I have tissues which were fixed in glutaraldehyde and then embedded in
paraffin.
Does anyone have experience in performing IHC on sections processed this way?
Routine methods (antigen retrieval with citrate buffer etc.) do not seem to
produce good results.
The tissue were originally designated for EM.
Thanks,
PKwan in Boston
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