FW: Cyclin D1 antibody

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From:"Brennan, Liam" <Liam.Brennan@bll.n-i.nhs.uk>
To:HISTONET <histonet@pathology.swmed.edu>
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Hi Jim- thanks for your input, Cell Marque is not a company I am
familiar with. Do you have an address/e-mail/Web Site/phone no. for them
or do you know who their distributors are in the UK or Ireland.
						- Liam

				Liam Brennan
				Histopathology Dept.
				Belfast City Hospital
				Northern Ireland
> ----------
> From: 	burch007@mc.duke.edu[SMTP:burch007@mc.duke.edu]
> Sent: 	28 June 2000 12:34
> To: 	Brennan, Liam
> Subject: 	Re: Cyclin D1 antibody
> 
> 
> 
> Hi,
> 
> On FFPE and B-5FPE sections, I use the polyclonal cyclin D-1 from Cell
> Marque
> with Dako Target  Retrieval pH 6.0 solution, 5 minutes of pressure in
> the
> BioCare DeCloaker chamber. Link and label is with Zymed Laboratories
> HistoStain
> Plus system. Very good results. Consistant. One key is using fresh cut
> sections,
> usually not an issue with daily cases, but positive control slides can
> go bad
> over time. Rumor has it Cell Marque gets their antibody from Santa
> Cruz
> Biochemicals.
> 
> Jim Burchette
> Duke Medical Center
> Immunopathology Lab
> 
> 



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