Here's our modified procedure:

slides to water
routine hematoxylin, differentiate, blue, etc.
0.02% fast green for 5 minutes
rinse
1.0% glacial acetic acid, 3 dips
0.1% saf O, 10 minutes
rinse
dehydrate
clear
coverslip



Jim

  _________________________
  James E. Staruk, HT(ASCP)
   Mass Histology Service
(A division of DVMLab, Inc.)
   www.masshistology.com

-----Original Message-----
From: CYNTHIA HAYNES [mailto:cynthiahaynes@hotmail.com]
Sent: Tuesday, February 25, 2003 11:29 AM
To: histonet@pathology.swmed.edu
Subject: Methyl green-Saffrain-o



Hello my fellow histonetters,
        Does any one have a protocol for Fast green(methyl green)-Saffrain-o
stain for paraffin on decaled bone. The procedure I am using is not working
for some strange reason. If any has a procedure for making up the solutions
I would greatly appreciated.
                                      Thanks in Advance
                                    Cynthia Haynes H.T.

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