Atrogin 1 in e14 rat skeletal muscle: FFPWS using ECM Bioscience antibody AP2041 Image January 2, 2015 Carl Hobbs T/S of developing skeletal muscle shows positivity at junction of primary/secondary myotubes ( black arrowheads). Red arrowheads indicate blood vessel smooth muscle positivity. I have no idea if this is “true” positivity. I welcome comments.