NeuN in goat brain : FFPWS using Abcam ab177487 Image August 30, 2017 Carl Hobbs As in all species I have tested , Purkinje cells of the cerebellum are always negative for this Ab.
NeuN in rat brain : FFPWS using Abcam ab177487 Image August 30, 2017 Carl Hobbs Arrowheads indicate the negative rostro-migratory stream ( RMS) of the progenitor olfactory neurones.
NeuN in mouse brain : FFPWS using Abcam ab177487 Image August 30, 2017 Carl Hobbs Note the positive post-mitotic neurones and the negative progenitor olfactory interneurones in the svz of the lateral ventricle. All neurons, as far as I am aware, are HuC/D positive.
Mast cells in mouse skin: FFPWS using 1% Toluidine blue ( aq) Image August 20, 2017 Carl Hobbs Same method as in FS of rat bladder
Mast cells in rat bladder: FS using 1% Toluidine blue ( aq) Image August 20, 2017 Carl Hobbs I overstained the tissue section and then dipped it into 0.5% HCL in 20% IMS to differentiate the staining intensity until I was happy with the signal: noise. It is a tad more laborious than using more highly selective methods but, serves a purpose.