Cleaved caspase 3 in mouse neurons: FFPWS using Cell Signalling’s 9661

CC3_(DAPI+Alexa-568)

Primary cultured neurons induced to apoptose by addition of Amyloid beta. The cell on the left is fragmenting and contains no nucleus ( negative for Hoechst). The cell on the right is in early stages of apoptosis as it still has a Hoechst-stained nucleus.

Cells were grown in matrigel. Fixed and processed to Pwax.

Note many Hoechst-positive small objects: most likely the culture was contaminated and these are bacteria.

Comments welcome.