CGRP has been tagged with GFP.
Positivity is localised to Lamina I?
( entry zone for Dorsal root nerves)
CGRP has been tagged using GFP. Localisation is identical to that of Abcam’s chicken anti CGRP antibody.
Citric acid HIER gave excellent results.
I do not use HCL +/- Trypsin anymore as it is too variable imho.
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