T/S at region of dorsal root entry.
All posts by Carl Hobbs
Nestin in rat e16 heart: FFPWS using DSHB Nestin 401 antibody
Claudin 3 in rat spinal cord: FFPWS using Labvision 9251
Claudin 3 in rat ganglion: FFPWS using Labvision 9251
NCAM-PSA in mouse e18 kidney: FFPWS using sigma C9672
beta III tubulin in Mouse e18: FFPWS using abcam ab14545
NF160 in mouse e18 embryo T/S: FFPWS using Sigma N-5264
beta III Spectrin in Mouse e14: FFPWS using Santa cruz sc-9660
Calbindin D Parvalbumin and S100: FFPWS of adult mouse cerebellum
CD4 Microwave V 90c Citric acid Antigen retrieval: FFPWS using abcam anti CD4 ab183685
Mouse spleen.
Upper image after M/W ( 1/2000). Should be further diluted.
Lower image after 90C AR for 60mins ( 1/1000)
M/W gives superior results and a higher dilution factor.
However, AR at 90C gives an identical pattern of positivity.
AR at 90C for 30mins gave an identical pattern but, was approx. half as intense as 60 mins AR ( not shown)