Hippocampus
A punctate positivity is seen( arrowheads) on ganglion cells ( black), associated nerve fibres ( red) and nerve fibres within the smooth muscle layers ( green).
I used TRIS pH9+EDTA HIER solution.
Identical positivity after TRIS pH9 ( no EDTA).
After Citric acid pH6 HIER, positivity was identical.
However, background noise was seen ( decreased Signal:noise)
TRIS pH9 + EDTA HIER.
Omitting EDTA gave identical positivity.
Citric acid pH6 HIER.
No nuclear positivity is seen.
NB: compare with image of rat e14 hypertrophic cartilage.
Positivity is localised to nuclei of developing respiratory tree epithelium.
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