hFGF-20 inrat brain lateral ventricle’s svz: FFPWS using R&D MAB2547

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The antibody is either of low sensitivity or the protein is very low copy-number because the positivity is moderate using a std stABCpx-DAB detection ( lower image). Using Nova red kit increased the signal significantly

Of course, one may use the higher sensitive DAB kits or, add  imidazole to your DAB solution.