Dear Irene

here the recommendations for anti-caspase 3 staining from David.

Best regards

Michael


David's recommendations:

We just used Tris Buffer at ph10 in the microwave. You will need to figure
out your own timing but the trick is to boil for 20 mins. No real trick
here it's just a matter of being able to open up those determinents. I have
not tried other methods but I'm sure other methods would work as well if
not better. When I spoke with a tech at Pharmigen thay told me they haven't
been able to get it to work on formalin fixed tissue...Hmmmmm!!! We decided
to give it a shot and from what we can tell there is some real staining.
Good Luck!

Michael,

Your welcome. I can't tell you. I sent my slides to the histochem lab for
them to run it thru their ag. ret. method since they have the timing all set
up. I cheated sorry.
Our control primarily were those cell undergoing granular vacuolar
degeneration which has been reported in literature to be associated with
Caspase 3 activity.

Back to you,

David

Michael Stumm, MD, Lab 405
Department of Research
Kantonsspital Basel
Hebelstrasse 20
4031 Basel
Switzerland
phone: ++41/61/265 23 84
email: stumm@ubaclu.unibas.ch