A note about Permanent Red.  The spec sheet recommends bring the reagents to
room temperature before dissolving the tablet in the substrate buffer. In
our hands this is a must.  When we've forgotten to bring the reagents to
room temp, and went ahead and dissolved the tablet and used the reagents,
the staining was patchy or absent.  We routinely pull the necessary amount
of tablets and substrate buffer out of the fridge 30 minutes prior to the
Dako Autostainer Batch step.  The staining has been great.  

Tim Coskran 
Pfizer 


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