Sue, We use clear rite on our stainer with no problems, I have 3 changes in 
the front, 100, 95, 80 then h2o. Then 3 on the end, we have a sakura 
coverslipper so we  set the slides in xylene before going on the machine.  I 
would like to try clear rite on the processor but have not done it yet.  
Hope this helps.  If we are running Immunos we leave the slides in the clear 
rite for 5 minutes, otherwise it is 3 minutes each.
Anita Dudley
Providence Hosp.
Mobile, al.

>From: Sue Barnes <SBarnes@elch.org>
>To: "'HISTONET@PATHOLOGY.SWMED.EDU'" <HISTONET@PATHOLOGY.SWMED.EDU>
>Subject: XYLENE SUBSTITUTE
>Date: Thu, 21 Sep 2000 13:45:55 -0400
>
>Our lab is trying to go with a xylene substitute for both processing and
>staining.  at the present time we are trying Clear Rite by Richard Allen.
>The processing appears to be fine but we are having problems with the
>staining.  Does anyone have a staining protocol using clear rite?  Could 
>you
>please send the protocol along with the changing reagent cycles that are
>working for you.  We never had these problems with xylene.
>
>We are also wanting to switch to a formalin substitute.  which one is
>working the best?
>
>Does anyone know who makes Bio Clear.  this one was suggested to us.
>
>We have a Renaissance processor and a Leica Auto stainer XL.
>Thanks for any help.
>Sue
>

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