<underline>RE: Ayoub-Shklar method (LUNA - Histopathologic methods,
1992)



Here it is

-</underline> Dewax and bring to distilled water

- Stain in 5% Acid Fuchsin for 3 minutes

- Transfer slides to Aniline blue - orange G solution for 45 minutes

- Transfer slides to 95% Alcohol, 3 changes 1 minute each

- Dehydrate slides from 95% Alcohol, 3 changes absolute, 3  change
xylol 

- Mount


<underline>Aniline blue- Orange G solution

</underline>Water soluble Aniline blue - 0.5g

Orange G                         - 2.0g

Phosphotungstic acid        - 1.0g

Distilled water 				- 100mL




<bold><underline>Results</underline></bold>

keratin								- brilliant red

Stratified squamous epithelium		- gray

stratum spinosum (prekeratin)		- orange

connective tissue					- blue

erythrocytes						- red




Rosalba Zumbo

Technical Officer ( Scientific)

Institute of Forensic Medicine

Sydney, Australia