Hi Anita,

I have a copy of Thompson, which, on page 480 gives a metanil yellow
counterstain after PAS. The solution is: 0.25% metanil yellow in 0.25%
acetic acid prepared with distilled water. Mix, filter and store in a clean
brown bottle.
After conventional periodic acid and Schiff sequence, and water washing,
stain 1 minute in metanil yellow, rinse in distilled water, 95% ethyl
alcohol, absolute ethyl alcohol and xylene. Thompson suggests mounting with
Permount.

Hope this is some help.

Ken.

At 09:25 AM 10/6/99 -0700, Anita Jennings wrote:
> I need a procedure that is found on page 480 of "Thomas Histology" for PAS
>that conterstains yellow. Can anyone help? anita
>
>
>
Kenneth W Turner
Senior Teaching Fellow
Manager Histology Service Unit
Department of Pathology			
Dunedin School of Medicine	    e-mail  ken.turner@stonebow.otago.ac.nz	
University of Otago		    Phone:  (03) 479 7135 or (03) 479-7152
New Zealand			    Fax:    (03) 479-7136