a small pinch or eosin dye powder in the last alcohol on your processor will do the trick. Tissues will appear bright pink at the embedding station but the final stain will look just fine and your pathologists won't even know that you've used this trick.

by the way, mecurochrome is a mercury compound and is best not used because one could argue that other solutions on the processor become contaminated with mecury.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue
Suite 309
Charleston, SC  29425
ph:  (843) 792-6353
fax: (843) 792-8974
email: Dellav@musc.edu

>>> "Paula Wilder" <histo20@hotmail.com> 10/05/99 11:46AM >>>
Hello Histonetters,

We have discovered that there is no longer a source for mecurochrome.
We are looking for an alternative for dyeing our tissues for orientation at 
embedding.  The pathologists do not want to see any dye color under the 
scope.  Does anyone have any suggestions?  Any input will be greatly 
appreciated.  Thank you in advance.

Paula Wilder
St. Joseph Medical Center
Towson, MD.


______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com