Karen,
Some years ago I published a paper with a colleague at the University of
Georgia on automated skeletal staining. The reference is Miller, Doris M.
and JE Tarpley. An automated double staining procedure for bone and
cartilage. Biotechnic and Histochemistry, 71:2,79-83, 1996. The paper was
for mouse embryos, but you might find some ideas you could use with fish.
Best of luck.

John Tarpley 15-2-B
Associate Scientist
Specialist Image Analysis & Immunohistochemistry
Amgen Inc
One Amgen Center Drive
Thousand Oaks, CA  91320

Views expressed are mine alone and do not represent the views of my employer


> ----------
> From:
> larisonk@uoneuro.uoregon.edu[SMTP:larisonk@uoneuro.uoregon.edu]
> Sent: 	Thursday, October 07, 1999 9:48 AM
> To: 	HistoNet@pathology.swmed.edu
> Subject: 	Alizarin Red
> 
> Histonetters,
> 
> We plan to screen fish embryos for skeletal mutations using Alizarin Red 
> staining.  Does anyone have any ideas for ways to streamline this protocol
> 
> for high throughput.  We're always looking for ways to save time on our 
> screening protocols as we screen thousands of embryos.
> 
> Karen in Oregon
> 
>  
> 
>